12 signs you have an unhealthy gut.

  1. Diarrhea, loose stool, constipation, less than one bowel movement per day.
  2. Gas or bloating
  3. Nutritional deficiencies
  4. Poor immune system
  5. Headaches, brain fog, memory loss
  6. Fatigue
  7. Skin rashes and problems such as acne, eczema or rosacea
  8. Cravings for sugar or carbs
  9. Arthritis or joint pain
  10. Feel like you have inflammation
  11. Depression, anxiety, ADD, ADHD
  12. Autoimmune diseases such as rheumatoid arthritis, lupus, celiac disease or Crohn’s

What is the GI Effects Comprehensive Stool Profile?

GI Effects is an innovative stool test measuring premier biomarkers of gastrointestinal function, providing valuable clinical insight into digestive performance, gut inflammation, and the gut microbiome — areas affecting not only GI health, but overall health as well.

The sophisticated biomarkers from the GI Effects Comprehensive Profile are reported using an intuitive DIG framework, providing key clinical information for three main gastrointestinal functional areas:


    • Pancreatic Elastase-1, a marker of exocrine pancreatic function
    • Products of Protein Breakdown, markers of undigested protein reaching the colon
    • Fecal Fat, markers of fat breakdown and absorption

Zonulin is a protein modulator of intestinal tight junctions that is used to assess intestinal permeability (IP). Recent peer-reviewed literature highlights the role that intestinal permeability plays in a host of chronic GI and systemic conditions.

Persons who may benefit from Zonulin assessment include those with:

  • Leaky Gut Symptoms – bloating, gas, cramps, food sensitivities, joint pains, skin rashes and autoimmunity
  • Celiac Disease
  • GI Disorders – gluten sensitivity, IBS
  • Cardiometabolic Diseases – type 1 diabetes, type 2 diabetes, obesity, non-alcoholic fatty liver disease, and insulin resistance


    • Calprotectin, a marker of neutrophil-driven inflammation; produced in abundance at sites of inflammation, this biomarker has been proven clinically useful in differentiating between inflammatory bowel disease (IBD) and irritable bowel syndrome (IBS)*
    • Eosinophil Protein X, a marker of eosinophil-driven inflammation and allergic response
    • Fecal Secretory IgA, a marker of gut secretory immunity and barrier function
    • Additional biomarkers available: Fecal Lactoferrin.

Gut Microbiome:

    • Metabolic indicators, demonstrating specific and vital metabolic functions performed by the microbiota
      • Short-Chain Fatty Acids, a metabolomic indicator of GI microbiome health
      • Beta-glucuronidase, an inducible enzyme involved in the metabolism and bioavailability of food and drug compounds; also produced by gut bacteria
      • Commensal Bacteria, demonstrating the composition, diversity, and relative abundance of gut organisms, all of which are linked to both gastrointestinal and general health
      • More than 95% of commensal gut organisms are anaerobic and are difficult to recover by traditional (aerobic) culture techniques; molecular DNA techniques are now considered the standard for anaerobic bacteria assessment in research, permitting identification and quantification of multiple organisms with a single specimen.
      • The Polymerase Chain Reaction (PCR) methodology can identify bacterial populations at any level of taxonomy, as broadly as phylum and as narrowly as species. This ability permits analysis of the gut microbiome at a desired degree of complexity.
      • GI Effects assesses a key set of 24 clinically relevant genera/species that map to 7 major phyla.
      • Bacterial and mycological culture, which demonstrate the presence of specific beneficial and pathological organisms
      • Traditional bacterial culture complements DNA-based tests to provide an expanded survey of a person’s gut microbiota, beyond the specific organisms targeted by PCR.


      • GI Effects provides microscopic examination of fecal specimens for ova and parasites (O&P), the gold standard of diagnosis for many parasites.
      • Enzyme immunoassay (EIA), widely recognized for its diagnostic utility in the detection of pathogenic antigens, is used for the identification of Cryptosporidium, Entamoeba histolytica, and Giardia lamblia.
      • Determination of one-day or three-day sample collection is based on clinician’s clinical index of suspicion for parasitic infection. If no/low suspicion, a one day sample will likely be adequate. If high suspicion, a three day sample collection is optimal.

Additional biomarkers available:

    • Campylobacter EIA
    • Clostridium difficile EIA
    • Escherichia coli EIA
    • Helicobacter pylori Stool Antigen EIA

When should the GI Effects Comprehensive Stool Profile be considered?

GI Effects assesses three critical areas of gut health: digestive function, gut inflammation, and the gut microbiome—areas affecting not only GI health, but overall health as well. For persons with symptoms that may have gastrointestinal dysfunction as their root cause, comprehensive stool diagnostics provide an effective tool for gaining clinical insight into next steps.

In addition to performing critical digestive functions, the intestinal tract contains significant amounts of organisms — the gut microbiome. Imbalances in this internal ecosystem have been associated in the scientific literature with a wide variety of common illnesses including, but not limited to:

  • Irritable Bowel Syndrome (IBS)
  • Inflammatory Bowel Disease (IBD)
  • Poor Digestion, Gas, Bloating
  • Diabetes
  • Obesity, difficulty losing weight
  • Cardiovascular Disease
  • Celiac and Other Malabsorption Disorders
  • Anxiety, Depression
  • Suspect Autoimmune

Assessing and normalizing GI function can have profound effects on overall health, leading to improvement in these and other conditions. The GI Effects® Comprehensive Stool Profile is a critical clinical tool in the management of persons presenting with symptoms that can be associated with compromised gut health.

Functional testing can help uncover the root cause of many chronic conditions that often leave a person feeling frustrated. The scope of the premier biomarkers on the GI Effects Stool Profile provides comprehensive information for me, the clinician, for the development of strategic interventions. As identified functional imbalances and inadequacies become more normalized through targeted dietary, lifestyle, and supplementation therapeutics, intractable symptoms often improve for most persons.

Each biomarker is associated with specific pertinent therapies, but general therapeutic considerations include:

  • Discerning general evidence of bowel inflammation and providing intestinal mucosal and anti-inflammatory support
  • Addressing any identified infection with appropriate pharmacological and botanical treatments
  • Supporting commensal bacteria with pre/probiotic supplements and dietary changes

Call us today to get your gut testing underway!